DNA

Part:BBa_K3289014:Design

Designed by: Tatiana Houhou   Group: iGEM19_NYU_Abu_Dhabi   (2019-10-21)


ypo2088 for the Detection of Plague


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we ordered our gblocks from IDT, we requested that they are 5' end phosphorylated to facilitate blunt end ligation into the pET backbone vector which was then transformed into E coli. for DNA cloning.


Source

Yersinia Pestis

References

(1) Yersinia Pestis, web.uconn.edu/mcbstaff/graf/Student presentations/Y. pestis/Yersinia pestis.html.

(2) “Yersinia Pestis.” Microbewiki, microbewiki.kenyon.edu/index.php/Yersinia_pestis.

(3) Harvard Health Publishing. “Plague (Yersinia Pestis).” Harvard Health, www.health.harvard.edu/a_to_z/plague-yersinia-pestis-a-to-z.

(4) “Plague.” World Health Organization, World Health Organization, www.who.int/news-room/fact-sheets/detail/plague.

(5) Matero, Pirjo, et al. “Real-Time Multiplex PCR Assay for Detection of Yersinia Pestis and Yersinia Pseudotuberculosis.” APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, U.S. National Library of Medicine, Jan. 2009, www.ncbi.nlm.nih.gov/pubmed/19161535.

(6) Zhou, Dongsheng, et al. “Identification of Signature Genes for Rapid and Specific Characterization of Yersinia Pestis - Zhou - 2004 - Microbiology and Immunology - Wiley Online Library.” Microbiology and Immunology, John Wiley & Sons, Ltd (10.1111), 14 Nov. 2013, onlinelibrary.wiley.com/doi/10.1111/j.1348-0421.2004.tb03522.x/full.